Author:
Edition: 2011
Publisher: Humana Press
Binding: Hardcover
ISBN: 1617379662
Heterologous Gene Expression in E.coli: Methods and Protocols (Methods in Molecular Biology)
Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Medical books Heterologous Gene Expression in E.coli. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli Medical books Heterologous Gene Expression in E.coli (Hardcover). Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately...
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Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Pro
Humana Press 9781617379666 Heterologous Gene Expression in E.Coli: Methods and Protocols (2011 Edition) Description *Author: Evans, Thomas C., JR./ Xu, Ming-Qun/ Evans Jr, Thomas C. *Series Title: Methods in Molecular Biology (Hardcover) *Series Number: 705 *Binding Type: Hardcover *Number of Pages: 310 *Publication Date: 2010/12/02 *Language: English *Dimensions: 10.10 x 7.10 x 0.90 inches SKU: UBM9781617379666 Payment We accept payment via PayPal, Mastercard, Visa, American Express, Discover
format hardback language english publication year 04 12 2010 series methods in molecular biology subject mathematics sciences subject 2 life sciences general title heterologous gene expression in e coli methods and protocols vol 705 author evans thomas c jr editor publisher springer verlag publication date dec 01 2010 pages 310 binding hardcover edition 1 st edition dimensions 7 00 wx 10 00 hx 1 00 d isbn 1617379662 subject science life sciences biochemistry brand new hardcover all orders get
Protein expression in a heterologous host is a cornerstone of biomedical research and of the biotechnology industry. Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Pro
Medical Book Heterologous Gene Expression in E.coli
Despite the advanced state of protein expression technology improvements are still needed. For example, membrane proteins constitute a significant percentage of the total cellular proteins but as a class are very difficult to overexpress, especially in a heterologous host. The ideal host would have the ability to express any protein, with relevant post-translational modifications, and be as easy to work with as E. coli. In Heterologous Gene Expression in E. coli: Methods and Protocols, expert scientists intimately familiar with the relevant techniques offer chapters that greatly expand the utility of this expression host. The contributions in this detailed volume describe methods, for example, to successfully express proteins in E. coli that would otherwise form aggregates in this host, to add post-translational modifications, to incorporate non-standard amino acid residues or moieties into E. coli expressed proteins, to identify binding partners, and to express membrane proteins. Written in the highly successful Methods in Molecular Biology™ format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Heterologous Gene Expression in E. coli: Methods and Protocols seeks to familiarize the researcher with the myriad of E. coli expression strains available and move E. coli closer to that ideal of the perfect host.