Friday, May 27, 2011

Optical Imaging Techniques in Cell Biology

Optical Imaging Techniques in Cell Biology



Author: Guy Cox
Edition: 1
Publisher: CRC Press
Binding: Hardcover
ISBN: 0849339197



Optical Imaging Techniques in Cell Biology


Since the word microscopy was coined in 1656, the evolution of the instrument has had a long and convoluted history. Medical books Optical Imaging Techniques in Cell Biology. Plagued with problems of chromatic aberration, spherical aberration, and challenges with illumination and resolution, the microscope’s technical progression happened in a series of fits and starts until the late 19th century. After Ernst Abbe perfected the “how” of lens design, achieving the theoretical limit imposed by wavelength, there came a revolution in subject matter or “what” could be studied by microscope.

Covering the entire field of microscopy, Optical Imaging Techniques in Cell Biology provides an overview of the technical evolution of the microscope and explains how the basics of optical microscopy led to the most advanced techniques employed today. The author addresses a vast array of topics including optical contrasting techniques, fluorescence, confocal versus widefield microscopes, lasers as a light source, and digital imaging, as well as the correction of aberrations that might arise Medical books Optical Imaging Techniques in Cell Biology , 9780849339196. Optical Imaging Techniques in Cell Biology , ISBN-13: 9780849339196, ISBN-10: 0849339197

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Optical Imaging Techniques in Cell Biology , ISBN-13: 9780849339196, ISBN-10: 0849339197

Optical Imaging Techniques in Cell Biology, Second Edition

Optical Imaging Techniques in Cell Biology

Optical Imaging Techniques in Cell Biology, Second Edition covers the field of biological microscopy, from the optics of the microscope to the latest advances in imaging below the traditional resolution limit. It includes the techniquessuch as labeling by immunofluorescence and fluorescent proteinswhich have revolutionized cell biology. Quantitative techniques such as lifetime imaging, ratiometric measurement, and photoconversion are all covered in detail. Expanded with a new chapter and 40 new figures, the second edition has been updated to cover the latest developments in optical imaging tec



Medical Book Optical Imaging Techniques in Cell Biology



Plagued with problems of chromatic aberration, spherical aberration, and challenges with illumination and resolution, the microscope’s technical progression happened in a series of fits and starts until the late 19th century. After Ernst Abbe perfected the “how” of lens design, achieving the theoretical limit imposed by wavelength, there came a revolution in subject matter or “what” could be studied by microscope.

Covering the entire field of microscopy, Optical Imaging Techniques in Cell Biology provides an overview of the technical evolution of the microscope and explains how the basics of optical microscopy led to the most advanced techniques employed today. The author addresses a vast array of topics including optical contrasting techniques, fluorescence, confocal versus widefield microscopes, lasers as a light source, and digital imaging, as well as the correction of aberrations that might arise. Building on this foundation, he then examines more advanced techniques such as quantitative fluorescence, fluorescence resonant energy, three-dimensional imaging, high-speed confocal microscopy, non-linear microscopy, and stimulated emission depletion.

Delivering a truly comprehensive work encompassing the scope and breadth of the field, the author brings a new level of understanding to the student, technician, researcher, or investigator working in the fascinating realm of optical microscopy.



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